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European Advances in Nucleic Acid Purification and Amplification Technologies

DateJun, 2005
Pages0
Price / format€3805 / Hard Copy
€3428 / Site License
€4558 / Enterprisewide

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Abstract:
 Increased Demand for Nucleic Acid Isolation Products Attributed to Growing Molecular Diagnostic Testing
The growing need for a large number of high-quality isolations in the research community is leading to an increasing acceptance of nucleic acid separation technology in the clinical laboratory community. Desire for high-throughput sequencing has spurred advances in automated instrumentation which has resulted in further utilisation of this technology. Apart from commercial diagnostic laboratories that require automated, high-throughput nucleic acid isolation products, there is also demand for low- to mid-throughput instrumentation from smaller hospital laboratories that desire to process their valuable samples in-house.
This Technical Insights study provides an overview of emerging trends in nucleic acid purification and amplification technologies in Europe. A synopsis of the emerging technologies involved in the nucleic acid purification [deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and plasmid DNA] and amplification is included in this analysis. The various technologies covered in this research service are membrane-based purification, magnetic separation technology, purification reagents, chromatography-based purification, thermal cyclers, and fluorogenic probes.
Automation Enhances User Friendliness, Spurring Growth of Nucleic Acid Purification and Amplification Products
The incorporation of automated instrumentation in the research process can eliminate human errors, while increasing productivity and resource savings, explains the analyst. The implementation of such automated systems enables scientists to spend more time designing experiments and performing data analysis, rather than handling experimental samples.
Detection tests based on nucleic acid amplification are much more sensitive than those conducted on immunoassays. Due to this, there is a huge demand from end users for the automation of standard procedures in the amplification process. The completion of the Human Genome Project, expiry of the polymerase chain reaction (PCR) patents and kits which contain all the pre-prepared ingredients necessary to realize numerous reactions, are also likely to spur demand for nucleic acid purification and amplification technology.

A Shift in Focus from Sequencing to Functional Genomics Drives the Market
The shift from sequencing to functional genomics has increased the need for consistently pure nucleic acid samples and automated instrumentation products. Moreover, the availability of whole genome sequences has helped research achieve a detailed understanding of the genotype-to-phenotype relationship. Nucleic acid-based amplification kits and EU's In Vitro Diagnostic Directive (IVDD) are likely to increase sale of commercial kits, thus securing strong demand for these products in future.
Despite drawbacks such as high costs of instruments, popularity of traditional methods and the lack of technological diversity, the future of these products is secure, notes the analyst. This is due to the expansion of genomics and proteomics research in both academia and industry, necessitating the use of these instruments to deliver reproducible, precise and accurate results.










Table of contents:
 1. Executive Summary

- 1. Introduction

- - 1. Introduction to Nucleic Acid Purification and Amplification Technologies

- - 2. Synopsis of Key Findings and Trends

- 2. Scope and Methodology

- - 1. Scope and Segmentation of the Research Service

- - 2. Methodology

2. Emerging Technologies to Purify Nucleic Acids

- 1. Emerging Technologies to Purify DNA

- - 1. Automated Membrane-Based System to Purify Nucleic Acids

- - 2. ChargeSwitch Technology to Purify Nucleic Acids

- - 3. Increasing Automation Results in a Reliable and Robust Manner

- - 4. Technology to Obtain Higher DNA Yield from Blood Samples

- - 5. Magnetic Tool To Increase Productivity in Nucleic Acid Purification

- - 6. Kit for Rapid Small-Scale Isolation of Genomic DNA from Blood Leukocytes

- 2. Emerging Technologies to Purify RNA

- - 1. Polymer Coated Beads for mRNA Purification

- - 2. Reusable Magnetic Beads To Purify mRNA

- - 3. Reagent for Rapid Isolation of Intact Total RNA

- - 4. Compact Automated System for Flexible and Reliable RNA and DNA Isolation

- 3. Emerging technologies to Purify Plasmids

- - 1. Plasmid Purification Kit To Enable Faster Purification by Reducing Intermediate Steps

- - 2. Chromatography Column To Purify pDNA

- - 3. Kit To Minimize Endotoxin Contamination While Purifying pDNA

- - 4. System To Provide a Fast Simple and Cost-Effective Plasmid Purification Method

- 4. Influential Market Factors

- - 1. Industry Trends

- - 2. Competing Technologies

- - 3. Technology Evolution

- 5. Technology Adoption Factor Analysis

- - 1. Technology Accelerators

- - 2. Technology Roadblocks

3. Emerging Technologies in Nucleic Acid Amplification

- 1. Emerging Technologies to Amplify Nucleic Acids

- - 1. Real-Time Platform To Enable Accurate and Precise Quantitative PCR

- - 2. DNA Polymerase Generates Templates with Greater Accuracy and Speed

- - 3. Rolling Circle Gene Amplification Technique

- - 4. Personal Thermal Cycler to Offer Faster Heating Rate and Block Uniformity

- - 5. Isothermal Gene Amplification System

- - 6. Fluorogenic Probes for More Specific Quantitative PCR

- - 7. Transcription-Mediated Gene Amplification

- - 8. Nucleic Acid Sequence-Based Amplification Technology

- - 9. Strand Displacement Amplification System

- - 10. Branched DNA Amplification Technology

- 2. Influential Market Factors

- - 1. Industry Trends

- - 2. Application Trends

- - 3. Competing Technologies

- 3. Technology Adoption Factor Analysis

- - 1. Technology Hurdles

- - 2. Technology Accelerators

- - 3. Adoption Roadblocks

- - 4. Technology Restraints

4. Database of Key Industry Participants; Selected Patents & Glossary

- 1. Selected Patents

- - 1. European Patents for Nucliec Acid Purification

- - 2. European Patents for Nucliec Acid Amplification

- 2. Database of Key Industry Participants & Glossary

- - 1. Companies and Organizations

- - 2. Glossary

5. Frost & Sullivan 2005 Science & Technology Awards

- 1. Technology Leadership Award

- - 1. Award Description

- - 2. Award Recipient

- 2. Product Leadership Award

- - 1. Award Description

- - 2. Award Recipient

- 3. Technology Innovation Award

- - 1. Award Description

- - 2. Award Recipient

6. Critical Reference Tables

- 1. Decision Support Database Tables

- - 1. Total Healthcare Expenditure (1999-2006)

- - 2. Pharmaceutical R&D Expenditure (1999-2006)

- - 3. Biotech Companies (1999-2006)

- - 4. Biotech Employees (1999-2006)

- - 5. Government Biotech R&D Investment (1999-2006)

- - 6. Percent Government Biotech Investment (1999-2006)

- - 7. Biotech Private Investment (1999-2006)

- - 8. EPO Biotech Patents (1996-2000)




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