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Healthcare and drugs / REPORT INFORMATION

Bioassays

DateMay, 2002
Pages130
Price / format€856 / Hard Copy
€1177 / Electronic

€856 

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Abstract:
 Based on a 34-question survey, Bioassays is the fourth of six titles in the series, The Tools and Techniques of Drug Discovery, and specifically provides valuable insight into the two major types of bioassays employed in drug discovery. One of the present bottlenecks in drug discovery is in efficiently identifying drug targets for additional research. By employing bioassays in automated format, the characterization of a large number of drug targets can be streamlined. The objective of this report is to review the applications and use of in vitro protein assays and cell-based assays as well as the limitations researchers have experienced while using them. The needs of the pharmaceutical industry continue to evolve over time and have resulted in considerable changes in the field's scientific approaches to drug discovery. Advances in bioassays and related instrumentation, as detailed by the research community in Bioassays, will provide the means necessary to address these evolving needs.

The major objectives of this report are as follows:

Examine the use and applications of cell-based and in vitro protein assays
Determine the features of these assays that are influential in purchasing decisions
Identify the preferred suppliers of bioassay reagents and kits
Establish the parameters for quality in cell-based and in vitro protein assays
Understand -- from the user's perspective -- the level of satisfaction with current products and any unmet needs in the area of bioassays








Table of contents:
 Section 1. Executive Overview and Introduction
1-1 Among the Study’s Key Findings
1-2 Bioassays, High-Throughput Screening and Drug
Discovery
1-2 Innovations
1-3 Key Bioassay-Based HTS Innovations
1-3 Detection Methodologies
1-5 Bioassays for HTS
1-7 Evolution of Bioassays: Formats and Functions
Section 2. Study Methodology and Demographics
2-1 Objectives
2-1 Comments
2-2 Assumptions
2-3 Definition of Terms
2-4 Market Segment
2-4 Organization Size
2-4 Laboratory Size
2-4 Job Position
2-4 Geographic Region
2-5 Area(s) of Research
Section 3. Significant Findings
3-1 In Vitro Protein Assays
3-2 Cell-Based Assays
Section 4. Presentation of Survey Data
Product Use & Application
4-1 Current use of bioassays
4-4 Locations where bioassay experiments are
performed
4-6 Principle reason bioassays experiments are not
performed within own lab/group
4-8 Applications for which bioassays are used
4-10 Relative amount of time spent performing in vitro or
cell-based assays
In Vitro Protein Assays
4-12 Applications for which in vitro protein assays
are used
4-14 Detection method most commonly employed in
in vitro protein assays
4-16 Percentage of in vitro protein assays performed
using specific types of proteins
4-17 Average number of individual data points collected
from the in vitro protein assays performed per week
4-18 Future use of in vitro protein assays
4-20 Most influential features in decision to use one
in vitro protein assay over another
4-22 Most influential factor in decision to purchase one
in vitro protein assay over another
4-24 Most significant performance limitation of in vitro
protein assays
4-26 Operational problems encountered in using in vitro
protein assays
4-28 Most valuable improvement to in vitro protein
assays
4-30 Interest in automating in vitro protein assays
Cell-Based Assays
4-31 Type(s) of cell-based assays used
4-33 Application(s) for which cell-based assays are used
4-35 Detection method most commonly employed when
performing cell-based assays
4-37 Average number of days to perform a cell-based
assay
4-38 Average number of individual data points collected
from enzyme assays per week
4-39 Future use of cell-based assays
4-41 Most influential features in decision to use one
cell-based assay over another
4-43 Most influential factor in decision to purchase one
cell-based assay over another
4-45 Most significant performance limitation
experienced when using cell-based assays
4-47 Operational problems encountered in using
cell-based assays
4-49 Most valuable improvements to cell-based assays
4-51 Interest in automating cell-based assays
Technologies & Suppliers
4-52 “Top-of-mind” company/supplier for bioassays
4-53 Primary supplier of reagents and kits for bioassays
4-55 Familiarity with suppliers of reagents and kits for
bioassays
4-57 Estimated percentage of current total annual budget
for products/services is dedicated to bioassays
4-58 Estimated percentage of future total annual budget
for products/services is dedicated to bioassays
Section 5. Benchmarking Data
5-1 Stage of drug discovery research
5-2 Type of drug discovery research conducted
5-4 Disease focus of drug discovery research
5-6 Biological processes or states monitored
5-8 Techniques used
5-10 Automated systems used
5-12 Types of equipment used
5-13 “Top-of-mind” company for products and services
for drug discovery
Section 6. Appendices
6-1 Overall comments as to where suppliers should
focus their efforts
6-2 Cross-Tabulations of Survey Data
6-25 Related Reports




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